Genesee & Wyoming Inc. (G&W) is an American short line railroad holding company, that owns or maintains an interest in 122 railroads in the United States, Canada, Belgium, Netherlands, Poland, United Kingdom and formerly Australia. It operates more than 13,000 miles (21,000 km) of owned and leased track. G&W owns or leases 116 freight railroads organized in locally managed operating regions with 7,300 employees serving 3,000 customers. The company had its roots in the Class III Genesee and Wyoming Railroad, which began in 1899.

G&W's four North American regions serve 42 U.S. states and four Canadian provinces and include 113 short line and regional freight railroads with more than 13,000 track-miles. G&W's UK/Europe Region includes the U.K.’s largest rail maritime intermodal operator and second-largest freight rail provider, as well as regional rail services in Continental Europe. G&W subsidiaries and joint ventures also provide rail service at more than 30 major ports, rail-ferry service between the U.S. Southeast and Mexico, transload services, and industrial railcar switching and repair.